|Tipo di tesi||Tesi di laurea magistrale|
|Titolo||Studi di stabilità di cocaina e metaboliti in matrice ematica mediante LC-MS/MS|
|Titolo in inglese||Stability studies of cocaine and metabolites in ematic matrix by LC-MS/MS|
|Struttura||Dipartimento di Scienze della Vita|
|Corso di studi||CHIMICA E TECNOLOGIA FARMACEUTICHE (D.M. 270/04)|
|Data inizio appello||2017-11-06|
|Disponibilità||Accessibile via web (tutti i file della tesi sono accessibili)|
Il presente lavoro di tesi sperimentale, svolto presso il laboratorio di Tossicologia Forense dell’Università di Modena e Reggio Emilia, ha avuto come obiettivo lo studio della stabilità della cocaina e dei suoi metaboliti in matrice ematica.
This experimental thesis work, conducted at the Forensic Toxicology Laboratory at University of Modena and Reggio Emilia, aims to study the stability of cocaine and its metabolites in the blood matrix. The study was structured in two successive phases: in the first phase, the in vitro stability of cocaine and its metabolites on blank blood samples, spiked with the target analytes, was evaluated in order to compare the results with the stability data in the literature; In the second phase, 24 blood samples coming from subjects founded positive to cocaine and metabolites in previous investigations of 2016, were re-analyzed. All the analyzed samples underwent the same processing, that involves the precipitation of blood proteins and then the supernatant purification by SPE-Phree columns for removing the phospholipids. The next LC-MS / MS analysis was carried out at 40 ° C on a Synergi ™ Polar-RP 80 Å (150 x 2.0 mm, 4.0 μm) column with gradient elution and MRM (multiple reaction monitoring) data acquisition in positive ionization mode. For the quantitative analysis, matrix calibration curves were calculated using the deuterated analogs of the target analytes (cocaine - d3 and benzoylecgonine - d3). At the end of the study period (46 days), all in vitro sample untreated and treated with NaF (as a cholinesterase inhibitor), showed a remarkable drop in cocaine concentration, in agreement with literature data; While the real samples, re-analyzed after one year, pointed out a drop in cocaine level strongly influenced by inter-individual differences. The results obtained in this thesis work provide more information on the stability of the target analytes in blood samples. In the toxicological-forensic field, this is of primary importance for the correct interpretation of analytical data; this is particularly more true if we consider that samples are usually analyzed not shortly after being collected and that additional analysis or remote confirmation after several months are often required.