Riassunto analitico
Nano biosensing plays an important role in the research and development of new drugs, allowing the study of various parameters including pharmacokinetics and drug-protein interactions. In this thesis work, a novel transistor-based biosensor exploiting as active material hybrid structures made of carbon nanotubes and human serum albumin (HSA/CNT) was fabricated: the fabrication procedure is simple, fast, and green, since it requires the deposition of a drop of aqueous solution containing the HSA/CNT adducts, without the requirement of organic solvents or high temperature. The HSA/CNT-based transistor has been electrical characterized in liquid showing good performances and reproducibility. The sensitivity and specificity of this carbon nanotube electrolyte-gated transistor biosensor were tested against three non-steroidal drugs (i.e. paracetamol, aspirin, and ibuprofen) by monitoring the effects on the electrical response of the device varying the drug concentration in solution. The various tests were performed by monitoring the variations of current, transconductance and threshold voltage inside. The calculated dose curves allowed to extract thermodynamic parameter for the couples HSA-drug. The results obtained have shown that this type of biosensor allows to monitor and analyze the interaction between HSA immobilize on CNT with a level of precision comparable to other analysis methods.
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Abstract
Nano biosensing plays an important role in the research and development of new drugs, allowing the study of various parameters including pharmacokinetics and drug-protein interactions. In this thesis work, a novel transistor-based biosensor exploiting as active material hybrid structures made of carbon nanotubes and human serum albumin (HSA/CNT) was fabricated: the fabrication procedure is simple, fast, and green, since it requires the deposition of a drop of aqueous solution containing the HSA/CNT adducts, without the requirement of organic solvents or high temperature. The HSA/CNT-based transistor has been electrical characterized in liquid showing good performances and reproducibility. The sensitivity and specificity of this carbon nanotube electrolyte-gated transistor biosensor were tested against three non-steroidal drugs (i.e. paracetamol, aspirin, and ibuprofen) by monitoring the effects on the electrical response of the device varying the drug concentration in solution. The various tests were performed by monitoring the variations of current, transconductance and threshold voltage inside. The calculated dose curves allowed to extract thermodynamic parameter for the couples HSA-drug. The results obtained have shown that this type of biosensor allows to monitor and analyze the interaction between HSA immobilize on CNT with a level of precision comparable to other analysis methods.
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