|Tipo di tesi||Tesi di laurea magistrale|
|Autore||GENNA, VINCENZO GIUSEPPE|
|Titolo||Nerve Growth Factor (NGF), potenziale nel trattamento dei difetti della superficie oculare.|
|Titolo in inglese||Nerve growth factor (NGF), potential in the treatment of ocular surface defects.|
|Struttura||BIOSCIENZE E BIOTECNOLOGIE|
|Corso di studi||BIOTECNOLOGIE MEDICHE E FARMACEUTICHE (D.M. 270/04)|
|Data inizio appello||2012-07-16|
|Disponibilità||Accessibile via web (tutti i file della tesi sono accessibili)|
Gli epiteli corneali e congiuntivali umani sono costantemente rinnovati durante tutta la vita grazie alla presenza di cellule staminali residenti (SCs).
The human corneal and conjunctival epithelia are constantly renewed during the life-time due to the presence of resident stem cells (SCs). Several ocular surface diseases (burns, autoimmune, congenital, etc…) are characterized by altered tear film due to both reduced tears and/or decreased number of conjunctival goblet cells with decreased production of mucins necessary to maintain a stable ocular surface. Recent studies have shown that Nerve Growth Factor (NGF) has pleiotropic action on ocular surface, exerting trophic action and modulation of corneal healing. NGF eye drops promotes corneal healing in patients with neurotrophic keratitis suggesting that NGF exerts a specific action on ocular surface progenitor cells or SCs. The overall aim of the project is to verify whether NGF may have similar healing effect on human conjunctival epithelial cells and if this activity is linked to a recruitment and/or activation of ocular surface SCs or progenitor cells. In vitro studies were performed on human conjunctival primary cultures exposed to NGF and effects of Nerve Growth Factor were related to expression of stemness/proliferation markers (ΔNp63α, c/EBPδ, Bmi1) and differentiation markers (14-3-3σ, K19). This study revealed that NGF treatment promotes conjunctival stem cells proliferation and differentiation to goblet cells, maintaining unaltered stem cells content. These results might have direct relevance for basic research, clinical knowledge and pharmaceutical industry, since NGF may be used to treat goblet cell-deficient ocular surfaces, leading to potential therapeutic intervention in several disease.