|Tipo di tesi
|Tesi di laurea magistrale
|Progettazione e validazione di un microscopio a luce strutturata basato su tecnologia DMD
|Titolo in inglese
|Design and validation of a structured-illumination microscope based on DMD technology
|Dipartimento di Ingegneria
|Corso di studi
|Ingegneria Elettronica (D.M.270/04)
|Data inizio appello
|Accessibile via web (tutti i file della tesi sono accessibili)
La tesi tratta la progettazione, lo sviluppo e la validazione di un microscopio a luce strutturata a basso costo ed elevata flessibilità, basato su un proiettore commerciale in tecnologia DMD.
The thesis presents the design, development and validation of a low-cost and high-flexibility structured-light microscope, based on a commercial projector equipped with DMD technology. Structured illumination consents the enhancement of the acquired image, both reducing out-of-focus light contributions and raising the optical resolution, allowing to go beyond the physical diffraction limit found in traditional microscopes (super-resolution). Moreover, it permits the optical sectioning and the subsequent 3D reconstruction of the sample. The innovation in the present work is the exploitation of a commercial micro projector to produce the structured-illumination patterns. Such choice permitted to severely reduce both the overall cost and size, while obtaining a highly versatile system that allows the user to easily modify the illumination patterns. Moreover, the projection speed typical of the DMD technology permitted to minimize the image acquisition time, lowering the impact of mechanical vibrations and/or sample movements on the obtained image. The thesis work treated the entire design process, from the opto-mechanical layout to the development of the control and elaboration software, written in Python and provided with a graphical user interface. Realized system has then been tested and validated, both with calibration standards (nanometric fluorescent beads) and with real biological samples, such as red blood cells and osteoblasts. Obtained results confirmed the optical sectioning capability, with subsequent 3D reconstruction of the sample. Moreover, the capability of the system to implement super-resolution algorithms has been verified.