• Acquisizione
• CRISPR/Cas
• F. columnare
• Microbiologia
• proteine Cas

Abstract
Bacteria that have the CRISPR/Cas molecular complex are able to respond to phage and plasmid invasions by implementing a response that can be defined as adaptive. Although the knowledge on the evolutionary aspects and on the biotechnological applications of the system is increasing, several aspects still lack complete characterization. Among these, information relating to the effectiveness of the mechanism in the event of incomplete CRISPR/Cas loci. My experiment is part of wider research that aim to characterize the acquisition of spacers in Flavobacterium columnare in which one of the CRISPR locus, the VI B locus, is incomplete in the acquisition machinery. It seems that the bacterium exploits the presence of another complete CRISPR/Cas locus, the II C locus, for the acquisition of spacers. The purpose of my thesis is to show the actual presence of new spacers through DNA amplification and purification of the specific acquisition bands, to better define the results of the previous studies and further supporting the hypothesis that there is a dialogue between the various CRISPR/Cas systems.

Abstract Bacteria that have the CRISPR/Cas molecular complex are able to respond to phage and plasmid invasions by implementing a response that can be defined as adaptive. Although the knowledge on the evolutionary aspects and on the biotechnological applications of the system is increasing, several aspects still lack complete characterization. Among these, information relating to the effectiveness of the mechanism in the event of incomplete CRISPR/Cas loci. My experiment is part of wider research that aim to characterize the acquisition of spacers in Flavobacterium columnare in which one of the CRISPR locus, the VI B locus, is incomplete in the acquisition machinery. It seems that the bacterium exploits the presence of another complete CRISPR/Cas locus, the II C locus, for the acquisition of spacers. The purpose of my thesis is to show the actual presence of new spacers through DNA amplification and purification of the specific acquisition bands, to better define the results of the previous studies and further supporting the hypothesis that there is a dialogue between the various CRISPR/Cas systems.