Riassunto analitico
Regenerative medicine is the main subject of scientific researches and it becomes increasingly meaningful due to the need to regenerate damaged age-related tissues, degenerative diseases, genetic defects or tumours.
Advanced Regenerative Therapies are concentrated on Stem Cells which can be used for direct application to damaged sites, for cell therapy, or for tissue engineering, using appropriate scaffolds as carriers for these cells.
The application of stem cells is considered to be more advantageous than that of the use differentiated cells. The stem cells are obtained more easily and in larger quantities, they are equipped with higher proliferation capacity, withstand more passages, undergo senescence later, can be differentiated into a wide range of desired cell phenotypes, and, also, can support the vascularization of scaffolds.
Stem cells can be defined as units of organization of biological systems and are responsible for the regeneration and development of organs and tissues, they are capable of self-renewal and differentiation into multiple cell lineages. For examples, when Stem Cells are directly injected into damaged tissues or into organs in vivo, their regenerative effects are based mainly on the autocrine production of a wide spectrum of bioactive molecules, e.g. growth factors, cytokines, chemokines, antiapoptotic factors and immunomodulators in form of proteins of small RNA species. The bioactive molecules synthesized in stem cells are stored in Extracellular Vesicles (EVs), which can be collected from the media conditioned by stem cells and administered systemically or locally into damaged tissues.
In this research study will be highlighted the best method of cell-culture, isolation and purification to harvest the Extracellular Vescicles, starting from Adult Stem Cells derived from Adipose Tissue.
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Abstract
Regenerative medicine is the main subject of scientific researches and it becomes increasingly meaningful due to the need to regenerate damaged age-related tissues, degenerative diseases, genetic defects or tumours.
Advanced Regenerative Therapies are concentrated on Stem Cells which can be used for direct application to damaged sites, for cell therapy, or for tissue engineering, using appropriate scaffolds as carriers for these cells.
The application of stem cells is considered to be more advantageous than that of the use differentiated cells. The stem cells are obtained more easily and in larger quantities, they are equipped with higher proliferation capacity, withstand more passages, undergo senescence later, can be differentiated into a wide range of desired cell phenotypes, and, also, can support the vascularization of scaffolds.
Stem cells can be defined as units of organization of biological systems and are responsible for the regeneration and development of organs and tissues, they are capable of self-renewal and differentiation into multiple cell lineages. For examples, when Stem Cells are directly injected into damaged tissues or into organs in vivo, their regenerative effects are based mainly on the autocrine production of a wide spectrum of bioactive molecules, e.g. growth factors, cytokines, chemokines, antiapoptotic factors and immunomodulators in form of proteins of small RNA species. The bioactive molecules synthesized in stem cells are stored in Extracellular Vesicles (EVs), which can be collected from the media conditioned by stem cells and administered systemically or locally into damaged tissues.
In this research study will be highlighted the best method of cell-culture, isolation and purification to harvest the Extracellular Vescicles, starting from Adult Stem Cells derived from Adipose Tissue.
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