|Tipo di tesi||Tesi di laurea magistrale|
|Titolo||Valutazione in vivo dell’attività antiossidante dell’estratto di Prunus avium L. utilizzando Caenorhabditis elegans come organismo modello|
|Titolo in inglese||In vivo evaluation of the antioxidant activity of Prunus avium L. extracts using Caenorhabditis elegans as a model organism|
|Struttura||Dipartimento di Scienze della Vita|
|Corso di studi||FARMACIA (D.M. 270/04)|
|Data inizio appello||2020-04-08|
|Disponibilità||Embargo di 3 anni|
|Data di rilascio||2023-04-08|
La presente Tesi Sperimentale di Laurea Magistrale in Farmacia è il risultato di uno studio realizzato nel laboratorio del Prof. Celestino Santos-Buelga e della Prof.ssa Ana Ma González-Paramás, presso il Dipartimento di Nutrizione e Bromatologia della Facoltà di Farmacia dell’Università di Salamanca (Spagna), nell’ambito del progetto di mobilità Erasmus+ (coordinatrice Prof.ssa Federica Pellati).
This Pharmacy Degree Experimental Thesis is the result of a study carried out in the laboratory of Prof. Celestino Santos-Buelga and Prof. Ana Ma González-Paramás, at the Department of Nutrition and Bromatology of the Pharmacy Faculty at Salamanca University (Spain), as part of the Erasmus+ mobility project (coordinator Prof. Federica Pellati). To carry out this study, Moretta di Vignola cherries, belonging to the species Prunus avium L., an autochthonous variety of sweet cherry typical of the territory surrounding the city of Vignola in the province of Modena, were used. The first part of the study was focused on the preparation of an extract rich in phenolic compounds and their characterization, through Folin-Ciocalteau method and HPLC separation coupled to spectrophotometric and mass analysis. Following the optimization of the process, we obtained an extract rich in anthocyanins, and in minor extension flavonols and phenolic acids, whose main component was cyanidine rutinoside. After obtaining the dry extract, the in vivo antioxidant activity was evaluated using Caenorhabditis elegans as a model organism. The antioxidant capacity was determined by subjecting the nematodes to thermal stress, at a temperature of 35 °C for six and eight hours, assessing their resistance, understood it as the survival of the nematode under these conditions. Exposing the nematode to this temperature results in production of reactive oxygen species (ROS) and oxidative damage. In order to identify the concentration of the extract capable of producing increased resistant, screening was carried out at different concentrations in the range 2.5-100 µg GAE/mL. The concentration that resulted in a significant increase in the survival rate of the nematode to thermal stress was 5 µg GAE/mL. The most interesting data was obtained on the second day of adulthood after 8 hours of thermal stress, with a difference of 25% between treated and controlled (DMSO) worms. The other concentrations analysed showed also efficacy. Interestingly increasing up to twenty times the concentration of the tested extract did not have a toxic effect. Therefore, a general evaluation of the results obtained in this study showed that Moretta cherry extract, rich in anthocyanins, at a concentration of 5 µg GAE/mL represents a product to be taken into high consideration for the formulation of nutraceutical products intended for protection against oxidative stress.