|Tipo di tesi||Tesi di laurea magistrale|
|Titolo||Caratterizzazione dei fattori implicati nell’equilibrio neuroinfiammatorio in sanguisuga medicinale Hirudo medicinalis|
|Titolo in inglese||Characterization of factors implicated in neuroinflammatory balance in the medicinal leech Hirudo medicinalis|
|Struttura||Dipartimento di Scienze della Vita|
|Corso di studi||BIOLOGIA SPERIMENTALE E APPLICATA (D.M. 270/04)|
|Data inizio appello||2017-02-16|
|Disponibilità||Accesso limitato: si può decidere quali file della tesi rendere accessibili. Disponibilità mixed (scegli questa opzione se vuoi rendere inaccessibili tutti i file della tesi o parte di essi)|
|Data di rilascio||2057-02-16|
La sanguisuga medicinale (Hirudo medicinalis) è considerata un buon modello per lo studio degli eventi molecolari e cellulari che portano alla riparazione del sistema nervoso centrale (SNC).
Medicinal leech (Hirudo medicinalis) is a well-known model for study the molecular and cellular events that lead the reparation of the central nervous system (CNS). The peculiarity of model leech is that, in contrast with vertebrates, its CNS undergoes synapse regeneration as a natural and functional mechanism leading to the restoration of locomotion. When the leech CNS is injured, microglial cells migrate and accumulate at the site of lesion. This step it’s been demonstrated essential for neurons outgrowth after axonal damage as well as axonal regeneration. In mammals, microglial cells are generally described as CNS macrophages, the first line of defence in the case of inflammation or neuronal pathologies. recent evidences suggest that microglia don’t play only a critical role in the pathological states but it’s involved in many different physiological functions to maintain the homeostasis of the brain. This is why in this study we want to investigate two different aspects of the same phenomenon. First, characterization by immunofluorescence and by western blot of one of the possible subclasses of microglia involved in neural repair, evidencing one recognised marker of neuroprotective microglia in mammals: arginase 1 (Arg1). As a second aspect, we tried to shed some light on how neurons and microglia dialogue during the reparation process by analysing the functions of HmC1q (Hirudo medicinalis C1q) which has chemotactic properties that are important in the microglial cell recruitment at the point lesion release by neurons. To pursue these objectives we used immunofluorescence approach and molecular approach: semi-quantitative and quantitative PCR to see if, after stimulation, HmC1q gene is upregulated or downregulated.